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A parallel algorithm for finding small sets of genes that are enough to distinguish two biological states Genet. Mol. Biol.
Torres,Martha; Barrera,Junior.
GCLASS is an algorithm which explores small samples of two distinct biological states for finding small sets of genes, which form a feature vector that is enough to separate these two states. A typical sample is a set of 60 microarrays, 30 for each biological state, with several thousand genes. The technique consists of the following: a spreading model defined in the space of small sets of genes studied and centered in each feature vector considered; the designing of optimal linear classifiers under this spreading model; and ranking the designed classifiers, based on their error and robustness relative to the spreading. The feature vectors used in the best classifiers are considered the best feature vectors. Due to the great number of potential feature...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene expression; Classification; Parallel processing.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400034
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A patient presenting a 22q13 deletion associated with an apparently balanced translocation t(16;22): an illustrative case in the investigation of patients with low ARSA activity Genet. Mol. Biol.
Artigalás,Osvaldo; Paskulin,Giorgio; Riegel,Mariluce; Burin,Maira; Saraiva-Pereira,Maria Luiza; Maluf,Sharbel; Kiss,Andrea; Schwartz,Ida Vanessa D..
A 10-year-old speechless, mentally deficient male, with low arylsulfatase A (ARSA) activity, and presumably, methachromatic leukodystrophy, underwent genetic evaluation. As the clinical picture was not compatible with this diagnosisan ARSA gene and chromosome analysis were performed, showing the presence of a pseudodeficiency ARSA allele and a de novo apparently balanced t(16;22)(p11.2;q13) translocation. A deletion on the long arm of chromosome 22 encompassing the ARSA gene, as shown by FISH and array-CGH, indicated a 22q13 deletion syndrome. This case illustrates the importance of detailed cytogenetic investigation in patients presenting low arylsulfatase A activity and atypical/unspecific clinical features.
Tipo: Info:eu-repo/semantics/article Palavras-chave: 22q13 deletion; Apparently balanced translocation; ARSA gene; Arylsulfatase A pseudodeficiency; Metachromatic leukodystrophy.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300007
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A pecuária de corte no Brasil: a importância da fêmea bovina. Infoteca-e
AZEVÊDO, D. M. M. R..
A pecuária de corte constitui-se em uma atividade de grande importância para o Brasil, visto que o país detém o maior rebanho comercial de bovinos do mundo. Neste contexto, estima-se que as raças zebuínas representam cerca de 80% da composição genética do rebanho brasileiro, constituído por aproximadamente 160 milhões de cabeças.
Tipo: Separatas Palavras-chave: Gado de Corte.
Ano: 2007 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/69576
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A pesquisa com Eucalyptus e Pinus na regiao dos cerrados. Infoteca-e
MOURA, V. P. G..
Na regiao dos Cerrados, o comportamento de especies/procedencias de Eucalyptus e bastante variavel de acordo com as condicoes climaticas e edaficas do local do plantio e tambem de acordo com a origem do material genetico, com produtividade variando de 15 a 60 m3/ha/ano. E. pilularis tem melhor desempenho em cerrado de alta altitude (1000 m). Em altitudes mais baixas, este material apresenta problemas fitossanitarios e fisiologicos. E. grandis desenvolve-se melhor em chuvas. Procedencias de Atherton sao as mais indicadas; procedencias do sul da Australia, apesar de apresentarem as maiores taxas de crescimento ate os sete anos de idade, apos este periodo sao acometidas de disturbios fisiologicos, causando a morte de grande numero de plantas adultas. E....
Tipo: Capítulo em livro técnico (INFOTECA-E) Palavras-chave: Pines; Eucalypts; Cerrado; Eucalipto; Pesquisa; Pinheiro; Eucalyptus; Pinus; Research.
Ano: 1997 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/553462
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A phylogenetic analysis of Brycon and Henochilus (Characiformes, Characidae, Bryconinae) based on the mitochondrial gene 16S rRNA Genet. Mol. Biol.
Hilsdorf,Silva; Oliveira,Claudio; Lima,Flávio César Thadeo de; Matsumoto,Cristianne Kayoko.
The genus Brycon, the largest subunit of the Bryconinae, has 42 valid species distributed from southern Mexico to the La Plata River in Argentina. Henochilus is a monotypic genus, comprising a single species (H. wheatlandii) found in the upper Rio Doce basin. In the present study, partial sequences of the mitochondrial gene 16S were obtained for fifteen species of Brycon and for Henochilus wheatlandii. The results showed that the genus Brycon is paraphyletic, since Henochilus is the sister-group of B. ferox and B. insignis. The most basal species analyzed were the trans-Andean species B. henni, B. petrosus, and B. chagrensis.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phylogeny; Molecular systematics; Mitochondrial DNA; Fish evolution; Systematic.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200034
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A phylogenomic appraisal of the evolutionary relationship of mycoplasmas Genet. Mol. Biol.
Yotoko,Karla S.C.; Bonatto,Sandro L..
Several genomes of mycoplasmas have been sequenced and here we tried to retrieve the evolutionary relationships of nine species using a phylogenomic approach. Several methods were used to build phylogenetic trees based on protein sequence information, gene-order, and gene-content. We also utilized datasets composed of individual and concatenated sets of orthologous proteins, as well as with reduced unreliable alignment regions. Most of our results converge to a single topology, except for the trees built with both the maximum parsimony method and with the gene-order dataset. The gene-content dataset presented trees consistent with most nodes of the convergent tree, but in the gene-order dataset most internal branches were clearly saturated and unreliable....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phylogenomic; Mycoplasma; Phylogenetic methods; Maximum likelihood; Maximum parsimony.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200015
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A polymorphic microsatellite from the Squalius alburnoides complex (Osteichthyes, Cyprinidae) cloned by serendipity can be useful in genetic analysis of polyploids Genet. Mol. Biol.
Boto,Luis; Cunha,Carina; Doadrio,Ignacio.
A new microsatellite locus (SAS1) for Squalius alburnoides was obtained through cloning by serendipity. The possible usefulness of this new species-specific microsatellite in genetic studies of this hybrid-species complex, was explored. The polymorphism exhibited by SAS1 microsatellite is an important addition to the set of microsatellites previously used in genetic studies in S. alburnoides complex, that mostly relied in markers described for other species. Moreover, the SAS1 microsatellite could be used to identify the parental genomes of the complex, complementing other methods recently described for the same purpose.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellites; Hybridogenesis; Squalius alburnoides.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300025
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A porcine gene, PBK, differentially expressed in the longissimus muscle from Meishan and Large White pig Genet. Mol. Biol.
Yonggang,Liu; Sumei,Zhao; Hongbin,Pan; Shizheng,Gao.
An investigation of differences in gene expression in the longissimus muscle of Meishan and Large White pigs was undertaken, using the mRNA display technique. A fragment of one differentially expressed gene was isolated and sequenced, whereupon the complete cDNA sequence was then obtained by using the rapid amplification of cDNA ends (RACE). The nucleotide sequence of the gene is not related to any known porcine gene. Sequence analysis revealed that the open reading frame of this gene encodes a protein with 322 amino acids, thus displaying high sequence identity with the PDZ binding kinase (PBK) of eleven other animal species - dog, horse, cattle, human, chimpanzee, crab-eating macaque, rhesus monkey, rat, mouse, gray short-tailed opossum and platypus, so...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pig; PBK; MRNA differential display; RACE.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400017
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A preliminary survey of M. hyopneumoniae virulence factors based on comparative genomic analysis Genet. Mol. Biol.
Ferreira,Henrique Bunselmeyer; Castro,Luiza Amaral de.
Mycoplasma hyopneumoniae is the etiological agent of porcine enzootic pneumonia (PEP), a major problem for the pig industry. The mechanisms of M. hyopneumoniae pathogenicity allow to predict the existence of several classes of virulence factors, whose study has been essentially restricted to the characterization of adhesion-related and major antigenic proteins. The now available complete sequences of the genomes of two pathogenic and one non-pathogenic strain of M. hyopneumoniae allowed to use a comparative genomics approach to putatively identify virulence genes. In this preliminary survey, we were able to identify 118 CDSs encoding putative virulence factors, based on specific criteria ranging from predicted cell surface location or variation between...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Porcine enzootic pneumoniae; Virulence; Host-pathogen interaction; Antigen; Adhesin.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200012
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A prevalent alpha-proteobacterium Paracoccus sp. in a population of the Cayenne ticks (Amblyomma cajennense) from Rio de Janeiro, Brazil Genet. Mol. Biol.
Machado-Ferreira,Erik; Piesman,Joseph; Zeidner,Nordin S; Soares,Carlos A.G.
As Rocky Mountain Spotted Fever is the most common tick-borne disease in South America, the presence of Rickettsia sp. in Amblyomma ticks is a possible indication of its endemicity in certain geographic regions. In the present work, bacterial DNA sequences related to Rickettsia amblyommii genes in A. dubitatum ticks, collected in the Brazilian state of Mato Grosso, were discovered. Simultaneously, Paracoccus sp. was detected in aproximately 77% of A. cajennense specimens collected in Rio de Janeiro, Brazil. This is the first report of Paracoccus sp. infection in a specific tick population, and raises the possibility of these bacteria being maintained and/or transmitted by ticks. Whether Paracoccus sp. represents another group of pathogenic Rhodobacteraceae...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paracoccus; Rickettsia amblyommii; Amblyomma; Ixodidae ticks.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500021
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A program for representing and simulating population genetic phenomena Genet. Mol. Biol.
Nuin,Paulo A.S.; Otto,P.A..
The paper describes a computer program for representing and simulating population genetic phenomena, such as the distribution of gene and genotype frequencies under different mating systems (panmixia, inbreeding and assortative mating systems) and under influence of evolution factors (mutation, selection, gene flow and genetic drift). The program was written in Visual Basic (Microsoft, Inc.) and is able to run in any IBM-PC compatible computer running Windows 3.1 or later versions.
Tipo: Info:eu-repo/semantics/article
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100009
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A prolificidade e a produção ovina. Infoteca-e
MORAES, J. C. F.; SOUZA, C. J. H. de.
Fertilidade e prolificidade; Estratégias para incremento da taxa de natalidade; O exemplo da introgressão do alelo Booroola; A incidência de partos triplos em rebanhos nacionais registrados; A seleção assistida pelo genótipo para o alelo Vacaria; Famílias com potencial para identificação de ?novos? genes principais; Família Durasnal; Família Salto
Tipo: Documentos (INFOTECA-E) Palavras-chave: Reprodução Animal; Ovinocultura; Ovino; Genética Animal.
Ano: 2018 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1103034
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A proposal of a standardised nomenclature for terminal minute sister chromatid exchanges Genet. Mol. Biol.
Drets,Máximo E.; Santiñaque,Federico F.; Obe,Günter.
We described spontaneous minute sister chromatid exchanges (SCE) in telomeric regions of human and Chinese hamster ovary (CHO) chromosomes more than 10 years ago. These structures, which we called t-SCE, were detected by means of highly precise quantitative microphotometrical scanning and computer graphic image analysis. Recently, several authors using the CO-FISH method also found small SCEs in telomeric regions and called them T-SCE. The use of different terms for designating the same phenomenon should be avoided. We propose ter SCE as a uniform nomenclature for minute telomeric SCEs.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Minute telomeric SCEs; Chromosome nomenclature.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300007
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A putative RA-like region in the brain of the scale-backed antbird, Willisornis poecilinotus (Furnariides, Suboscines, Passeriformes, Thamnophilidae) Genet. Mol. Biol.
Lima,Jamily L.R. de; Soares,Fabricio A.; Remedios,Ana C.S.; Thom,Gregory; Wirthlin,Morgan; Aleixo,Alexandre; Schneider,Maria Paula C.; Mello,Claudio V.; Schneider,Patricia N..
The memorization and production of song in songbirds share important parallels with the process of speech acquisition in humans. In songbirds, these processes are dependent on a group of specialized telencephalic nuclei known as the song system: HVC (used as a proper name), RA (robust nucleus of arcopallium), LMAN (lateral magnocellular nucleus of the nidopallium) and striatal Area X. A recent study suggested that the arcopallium of the Sayornis phoebe, a non vocal learner suboscine species, contains a nucleus with some properties similar to those of songbird RA, suggesting that the song system may have been present in the last common ancestor of these groups. Here we report morphological and gene expression evidence that a region with some properties...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vocal learning; RGS4; Song nuclei; In situ hybridization.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300249
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A putative resistant DNA marker for wool yellowing susceptibility in sheep Genet. Mol. Biol.
Benavides,M.V.; Damak,S.; Maher,A.P..
An Australian Merino flock was screened for low (resistant) and high (susceptible) yellow predictive colour (YPC) breeding values in order to compare extreme individuals using the differential display of mRNA technique. One differentially expressed cDNA band was visualised only in the resistant group. This band showed no identity with the DNA sequences of public databases; however, they showed short homologies with three database sequences related to transmembrane signalling functions. The use of these candidate genes as DNA markers needs to be confirmed against sheep with a wide range of susceptibility to wool yellowing to verify the results.
Tipo: Info:eu-repo/semantics/article
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200017
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A raça Gir Mocha na região Nordeste do Brasil: estrutura genética populacional via análise de pedigree Arq. Bras. Med. Vet. Zootec.
Muniz,L.M.S.; Souza,L.A.; Barbosa,A.C.B.; Ambrosini,D.P.; Oliveira,A.P.; Carneiro,P.L.S.; Malhado,C.H.M.; Martins Filho,R.; Duarte,R.A.B..
Foram utilizados dados de pedigree de 2.558 bovinos da raça Gir Mocha nascidos no período de 1954 a 2005. As análises foram realizadas utilizando-se o programa Endog. Do total de animais estudados, 61,9%; 10,6% e 0,1% possuíam pedigree na primeira, segunda e terceira gerações, respectivamente.O número efetivo de rebanhos que forneceram machos reprodutores foi de 10,25 para pais e 3,87 para avôs, confirmando a baixa integralidade do pedigree. O número de animais fundadores foi de 975,5, e o número efetivo de fundadores de 141,34. O número de ancestrais na população referência foi de 924 animas, dos quais apenas 39 explicaram 50% da variabilidade genética da população.O coeficiente médio de relação foi estimado em 0,75%,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ancestrais; Endogamia; Fundadores; Genealogia.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000600035
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A raça Indubrasil no Nordeste brasileiro: melhoramento e estrutura populacional R. Bras. Zootec.
Carneiro,Paulo Luiz Souza; Malhado,Carlos Henrique Mendes; Martins Filho,Raimundo; Carneiro,Antonio Policarpo Souza; Silva,Fabyano Fonseca e; Torres,Robledo de Almeida.
Com o intuito de fornecer subsídios para programas de melhoramento, conservação e expansão da raça Indubrasil do Nordeste brasileiro, avaliaram-se o histórico evolutivo, as estimativas de parâmetros genéticos e a estrutura populacional da raça. Foram utilizadas informações do pedigree de animais nascidos no período de 1964 a 2006 e dados dos pesos ajustados aos 205, 365 e 550 dias de idade de bovinos nascidos a partir de 1976. As estimativas dos coeficientes de herdabilidade foram menores que as encontradas na literatura para os pesos ajustados nas três idades (P205: direta 0,11 ± 0,03 e materna 0,01 ± 0,03; P365: direta 0,16 ± 0,04 e P550: direta 0,15 ± 0,05) e os ganhos genéticos para as características decresceram no período avaliado (P205: -0,028...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endogamia; Ganho genético; Intervalo de geração; Tamanho efetivo; Tendência genética.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009001200006
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A rapid alternative technique for obtaining silver-positive patterns in chromosomes Genet. Mol. Biol.
Kavalco,Karine Frehner; Pazza,Rubens.
Silver nitrate chromosome staining to evidence nucleolar organizer regions (NORs) is a widely adopted methodology. The aim of the present work was to improve this technique, reducing the preparation time without decreasing the quality of the results. Microwave irradiation proved to be quite efficient and reliable for this purpose, as it allowed to identify Ag-NORs equivalent to those obtained by the conventional procedure and also to reduce the concentration of the employed reagents, as well as the precipitation of debris on the preparation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Silver staining; Ag-NOR; Microwaves; Chromosome banding.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200012
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A rapid detection method for PAI-1 promoter insertion/deletion polymorphism (4G/5G) Genet. Mol. Biol.
Annichino-Bizzacchi,Joyce M.; Pugliese,Luciana; Arruda,Valder R..
Plasminogen activator inhibitor-1 (PAI-1) is an important inhibitor of fibrinolysis, and increased levels of PAI-1 are associated with atheroma and myocardial infarction. A common 4G/5G insertion/deletion polymorphism located in the promoter region of PAI-1 gene has been described associated with PAI-1 activity in plasma levels. Genotyping of this polymorphism is commonly conducted with an allele-specific oligonucleotide melting technique. In the present study, we describe a quick, easy method for genotyping 4G/5G polymorphism in the promoter region of the PAI-1 gene.
Tipo: Info:eu-repo/semantics/article
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300004
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A rapid technique for analysis of formalin-fixed, paraffin-embedded tissues by fluorescent in situ hybridization with alpha-satellite probes Genet. Mol. Biol.
Barril,Nilce; Carvalho-Salles,Andréa Borduchi; Tajara,Eloiza Helena.
We describe a rapid procedure for preparing archival tissues for interphase FISH analysis. The present protocol differs from others previously described because it allows the obtention of nuclei in satisfactory number and quality without using special equipments, adhesive-treated slides or solutions for chromatin decondensation. The method is of low cost and useful for retrospective analyses of formalin-fixed, paraffin-embedded samples.
Tipo: Info:eu-repo/semantics/article
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400006
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